ABSTRACT
PURPOSE: To investigate synergistic suppression of donor liver pre-perfusion with recipient serum (RS) and cobra venom factor (CVF) treatment on hyperacute rejection (HAR) following liver xenotransplantation. METHODS: Guinea-pigs (GP, n=24) and Sprague-Dawley rats (SD, n=24) were recruited. Before transplantation, serum was collected from SD rats and used for preparation of inactivated complements. GP and SD rats were randomly assigned into four groups (n=6), respectively: RS group, CVF group, RS+CVF group and control group. Orthotopic liver xenotransplantation was performed with modified two-cuff technique. The survival time and liver function of recipients, morphological and pathological changes in rat livers were investigated. RESULTS: There was no piebald like change in the recipient livers in all experiment groups. The survival time of recipients in all experiment groups was longer than that in control group (p<0.05). Moreover, the survival time in the RS+CVF group was markedly longer than that in the RS group (p<0.01) and CVF group (p<0.05). The serum ALT level in all experiment groups were lower than that in the control group (p<0.05). Furthermore, the ALT level in the RS+CVF group was significantly lower than that in the CVF group (p<0.05) and RS group (p<0.01). The histological damages were significantly improved when compared with the control group, and the histological damages in the RS+CVF group were milder than those in the remaining groups (p<0.05) CONCLUSION: Pre-perfusion of donor liver with recipient serum and cobra venom factor treatment can exert synergistic suppressive effects on the hyperacute rejection following liver xenotransplantation.
OBJETIVO: Investigar a supressão sinérgica da pré-perfusão do doador de fígado com soro do receptor (SR) e tratamento com fator veneno de cobra (FVC) na rejeição hiperaguda (RHA) após o xenotransplante de fígado. MÉTODOS: Foram utilizados Cobaias (GP, n=24) e ratos Sprague-Dawley (SD, n=24). Antes do transplante foram coletadas amostras de soro dos ratos SD e usados para a preparação dos complementos inativados. Cobaias GP e ratos SD foram randomicamente distribuídos em quatro grupos (n=6), respectivamente: grupo RS, grupo FVC, grupo SR+FVC e grupo controle. Xenotransplante ortotópico do fígado foi realizado com a técnica de dois cuffs modificados. Foram investigados o de tempo de sobrevida, a função hepática dos receptores e alterações morfopatológicas em fígados de ratos. RESULTADOS: Não houve alteração na coloração do parênquima dos fígados nos receptores. O tempo de sobrevida dos receptores em todos os grupos experimentais foi mais longo do que o grupo controle (p<0,05). Além disso, o tempo de sobrevida do grupo SR+ FVC foi marcadamente maior do que o grupo SR (p<0,01) e o grupo FVC (p<0,05). O nível sérico ALT foi menor em todos os grupos experimentais do que o grupo controle (p<0,05). O nível de ALT no grupo SR+ FVC foi significantemente menor do que no grupo FVC (p<0,05) e o grupo SR (p<0,01). As alterações histológicas foram significantemente melhoradas quando comparado com o grupo controle, e os danos histológicos no grupo SR+ FVC foram mais moderados do que nos grupos restantes (p<0,05). CONCLUSÃO: Pré-perfusão do fígado doador com soro do receptor e fator veneno de cobra pode exercer efeito supressor sinérgico da rejeição hiperaguda após xenotransplante de fígado.
Subject(s)
Animals , Female , Guinea Pigs , Rats , Blood Transfusion , Elapid Venoms/therapeutic use , Complement Inactivating Agents/therapeutic use , Graft Rejection/prevention & control , Graft Survival/drug effects , Liver Transplantation/physiology , Transplantation, Heterologous , Drug Evaluation, Preclinical , Graft Rejection/immunology , Graft Survival/immunology , Liver Transplantation/immunology , Liver Transplantation/mortality , Perfusion , Random Allocation , Rats, Sprague-Dawley , Transplantation, Heterologous/immunology , Transplantation, Heterologous/mortality , Transplantation, Heterologous/pathologyABSTRACT
It has been reported that the immune response due to alpha-Gal epitopes is an important factor in tissue valve failure. The elimination of the interaction between the natural anti-Gal antibodies and alpha-gal epitopes on the xenografts is a prerequisite to the success of xenografts in humans. Previously, we reported that the green coffee bean alpha-galactosidase could remove all alpha-Gal epitopes from cell surface of porcine aortic valve and pericardial tissue, but it has limitations on cost effectiveness. In this study we wanted to know whether the recently produced recombinant human alpha-galactosidase A has the same effective enzymatic activity as green coffee bean alpha-galactosidase in removing alpha-Gal epitopes from the same tissues. After treating fresh porcine aortic valve and pericardial tissue with recombinant alpha-galactosidase A, each sample was stained with Griffonia simplicifolia type I isolectin B4 indirect immunoperoxidase avidin-biotin technique. We then examined whether the alpha-Gal epitopes were reduced or abolished in each consecutive concentration of recombinant alpha-galactosidase A by comparing the degree of the Griffonia simplicifolia isolectin B4 staining. As a result, the recombinant alpha-galactosidase A could remove cell surface alpha-Gals on porcine aortic valve and pericardial tissue as effectively as green coffee bean alpha-galactosidase.
Subject(s)
Adolescent , Animals , Child , Humans , Aortic Valve/chemistry , Coffea/enzymology , Epitopes/immunology , Heart Valve Prosthesis Implantation , Pericardium/chemistry , Recombinant Proteins/genetics , Swine , Transplantation, Heterologous/immunology , alpha-Galactosidase/geneticsSubject(s)
Humans , Animals , Mice , Biotechnology , Transplantation Chimera/immunology , Transplantation, Heterologous/adverse effects , Transplantation, Heterologous/physiology , Transplantation, Heterologous/immunology , Transplantation, Heterologous/methods , Transplantation, Heterologous/psychology , Transplantation, Heterologous , Graft Rejection , Haplorhini , Mutation/genetics , SwineABSTRACT
Se presenta la experiencia del trasplante de Islotes de Langerhans de ratón a un sistema de derivación ventriculoperitoneal previamente instalado en otra especie (llama). Se plantea que el Líquido cefalorraquídeo (LCR) es un ambiente privilegiado desde el punto de vista inmunitario, en el sentido de carecer de tasas elevadas de anticuerpos y células inmunoactivas. El objeto del trabajo es demostrar la variabilidad a largo plazo y la funcionalidad de estos islotes dentro del ambiente de LCR del receptor. Se observó producción de insulina durante todo el tiempo de observación sin existir indicios de rechazo inmunológico. Se discuten las implicancias y posible desarrollo posterior de este tipo de experiencias
Subject(s)
Animals , Mice , Islets of Langerhans Transplantation , Cerebrospinal Fluid/immunology , Camelids, New World/cerebrospinal fluid , Culture Media/analysis , Diabetes Mellitus, Type 1/immunology , Transplantation, Heterologous/immunologySubject(s)
Humans , Dogs , Cricetinae , Mice , Rabbits , Rats , In Vitro Techniques , Graft Rejection/immunology , Transplantation Immunology/immunology , Transplantation, Heterologous/immunology , Antibody Formation , Species Specificity , Haplorhini , Immunity, Cellular , Immunosuppression Therapy , Pan troglodytes , Swine , Transplantation, Heterologous/trendsABSTRACT
El Banco de Huesos representa una gran ventaja para el paciente ortopédico en la práctica diaria. A partir de Octubre de 1985, se inició en el Hospital Universitario del Valle el desarrollo de un Banco de Tejidos del cual se han beneficiado pacientes de los Hospitales Universitarios del Valle y de Caldas. El presente es un trabajo de tipo multicéntrico, prospectivo y descriptivo, en el cual presentamos la experiencia obtenida con los primeros 22 pacientes, 9 hombres y 13 mujeres, con edad promedio de 29.5 años y seguimientos que oscilan entre los 7 y 10 meses(promedio de 8.5 meses). Durante el seguimiento no se ha detectado ninguna complicación relacionada con el trasplante de tejidos. El hueso de Banco ha permitido reducir en forma muy considerable el tiempo y la pérdida sanguínea en la cirugía de columna. Concluimos que la criopreservación de tejidos es una técnica útil que puede implementarse en nuestro país